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詳細介紹
Medium 199 培地是根據 Morgan、Morton 和 Parker 的合成培養基配方，經過島津特殊的制備方法調制而成的粉末培養基。
該培養基包含各種氨基酸、維生素、核酸成分和少量的中間代謝物等。單獨使用該培養基不能期望細胞增殖，但在脊髓灰質炎疫苗和其他病毒研究中被廣泛用作維持細胞的培養液。此外，添加血清后，該培養基在初代培養的細胞增殖方面非常出色。
稱取 9.5g 該粉末培養基溶解于蒸餾水中，制成 1000mL 溶液（禁止加熱）。加入適量（加入 1.3-2.2g 后，在 5% CO? 下，37℃時 pH 值為 7.1-7.4），溶解后立即進行過濾滅菌。預先使用 CO? 氣體調節 pH 值后再進行過濾可以防止過度的 pH 升高。如不立即使用，應密封保存在陰涼處（2-10℃）。在使用前，根據需要加入適量的血清。
該粉末培養基需要密封保存，并放置在干燥陰涼避光處（2-10℃）。
生產之后一年。
每 9.5g (1 L)
Sodium Chloride................................ 6,800 mg
Potassium Chloride............................. 400 mg
Calcium Chloride(Anhydrate).................. 200 mg
Magnesium Sulfate(Anhydrate)................ 97.7 mg
Sodium Dihydrogen Phosphate(Monohydrate).... 125 mg
Iron(III)Nitrate(Enneahydrate)................ 0.72 mg
Sodium Acetate................................. 50 mg
Adenosine 5' Triphosphate Disodium........... 1 mg
Glucose.......................................... 1,000 mg
L-Arginine Hydrochloride...................... 70 mg
L-Histidine Hydrochloride Hydrate............ 21.9 mg
L-Lysine Hydrochloride........................ 70 mg
L-Tryptophan.................................... 10 mg
L-Phenylalanine............................... 25 mg
L-Methionine................................... 15 mg
L-Serine........................................ 25 mg
L-Threonine..................................... 30 mg
L-Leucine...................................... 60 mg
L-Isoleucine................................... 20 mg
L-Valine........................................ 25 mg
L-Gutamic Acid................................ 66.8 mg
L-Aspartic Acid............................... 30 mg
L-Alanine....................................... 25 mg
L-Proline....................................... 40 mg
L-Hydroxyproline............................. 10 mg
Glycine......................................... 50 mg
L-Glutamine................................... 100 mg
L-Cystine...................................... 20 mg
L-Tyrosine...................................... 40 mg
L-Cysteine Hydrochloride Hydrate.............. 0.11 mg
p-Aminobenzoic Acid........................... 0.05 mg
D-Biotin........................................ 0.01 mg
Calcium Pantothenate.......................... 0.1 mg
Folic Acid...................................... 0.01 mg
myo-inositol................................... 0.05 mg
Nicotinic Acid................................ 0.025 mg
Nicotinamide................................... 0.025 mg
Pyridoxal Hydrochloride...................... 0.025 mg
Pyridoxine Hydrochloride...................... 0.025 mg
Riboflavin...................................... 0.01 mg
Thiamine Hydrochloride........................ 0.01 mg
Vitamin A Acetate............................ 0.1 mg
L-Ascorbic Acid............................... 0.05 mg
α-Tocopherol Disodium Phosphate............. 0.01 mg
Calciferol..................................... 0.1 mg
2-Methyl-1,4-Naphthokine..................... 0.01 mg
Adenine......................................... 0.9 mg
2-Deoxy-D-Ribose............................. 0.5 mg
Guanine......................................... 10 mg
Guanine Hydrochloride........................ 0.3 mg
Hypoxanthin................................... 0.3 mg
Thymine........................................ 0.3 mg
Uracil........................................... 0.3 mg
Xanthine........................................ 0.3 mg
AMP -2 Na..................................... 0.2 mg
ID-Ribose...................................... 0.5 mg
Glutathione................................... 0.05 mg
Cholesterol................................... 0.2 mg
Polysorbate 80............................... 5 mg
Phenol Red...................................... 6 mg
Other substances to be added
Sodium Bicarbonate.......................... As Needed
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